3P258 On phenotypic drug tolerance based on expression noise of antibiotic resistant gene(20. Origin of life & Evolution,Poster)
Takashi Nozoe, Reiko Okura, Yuichi Wakamoto · Seibutsu Butsuri · 2013
Chloroplasts in plants and algae show autofluorescence of chlorophyll a mainly from photosystem II, its light-harvesting complexes, and to a less extent from photosystem I and its antenna.The lifetime of fluorescence reflects photochemical reaction in chloroplasts.We have applied fluorescence lifetime imaging microscopy (FLIM) to chloroplasts in a green algae, in order to study fine structures of chloroplast in situ and their changes.We have also developed software to automatically analyze the results of FLIM data to extract fluorescence lifetime from grana thylakoid and stroma-exposed thylakoid separately.Three dimensional structures inside the chloroplasts and the dependence of the fluorescence lifetime on excitation laser intensity will be described.