A sensitive method for estimation of oxaloacetate
George Kalnitsky, Donald F. Tapley · Biochemical Journal · 1958
Finally, it should be pointed out that these methods have successfully been applied to studies of the anthocyanins present in colour mutants of Solanum phureja and Primula sinensi8, and in a variety of other plant material.Provisional structures for a number of newly discovered pig- ments have been advanced on the basis of spectral and chromatographic data, of identifying the sugars present (Harborne & Sherratt, 1957 a) and of examining the simpler glycosides produced by partial acid hydrolysis.Further details of these results will appear in later papers.SUMMARY 1. Spectral data for both the common and rare naturally occurring anthocyanidins are given.2. Spectral measurements have been made of a large number of anthocyanins.It is shown that 3-and 3:5-glycosides have characteristically different absorption properties in the 400-450 m, region.3. Measurements of the absorption spectra in the ultraviolet region of anthocyanins acylated with hydroxy aromatic acids can be used to deter- mine the hydroxy aromatic acid:pigment ratio in these complex pigments.4. The value of these measurements for characterizing anthocyanins is discussed.