Analytical Performance of Agilent Avida Duo with Custom Multiomic Reference Standards
Mike Ruvolo, Yingmin Wang, Yingzhen Wang, Heng Wang, Bonita R. Lam, Grace Zhao, Kyeong-Soo Jeong, Yun Bao, Shengrong Lin, Bram Herman, Mistuni Ghosh, Carole Durand · The Journal of Liquid Biopsy · 2025
Introduction: Interrogating multiple omics layers in cell-free DNA (cfDNA) using target enrichment next-generation sequencing (NGS) workflows offers significant value for high-sensitivity oncology research applications. Traditional approaches often require splitting samples across separate assays, potentially compromising sensitivity. Agilent Avida target enrichment technology enables efficient capture of native DNA fragments while preserving epigenetic features like methylation. This integrated method, known as Avida Duo, allows simultaneous analysis of both mutation and methylation status, maximizing data yield from a single cfDNA sample.