Membrane adsorber design for lentiviral vector recovery
George Pamenter, Danyal H Rahim, Ciaran M. Lamont, Maria Kapanidou, Kirstie Pemberton, Rui Sanches, Anurag Kulkarni, Oliver Charles Goodyear, Carol Knevelman, Kyriacos A. Mitrophanous, Andre Krause, Florian Taft, Volkmar Thom, Daniel G. Bracewell, Lee Davies · Molecular Therapy — Methods & Clinical Development · 2025
80% at t = 100 min). Narrower elution ranges were shown, with LV eluted <450 mM NaCl. The best-performing prototype, DR2, exhibited a ∼3-fold higher functional product recovery than standard Q-membranes for LV encoding GFP (50%) and CAR (73%) transgenes. At large-scale, downstream processes using DR2 membranes showed a 3.5-fold improvement in functional product recovery at drug substance (43%) compared to a standard Q-membrane process (12%). These results demonstrate that adsorbents designed for lentiviral vectors significantly enhance downstream recoveries.