Comparative antioxidant analysis using the iodine clock reaction: A kinetic approach
Hassan Darwish, Hassan Darwish, Evan Brewer, H. H. S. C. Rao, Julisa Laris, Hiba Al Lawati, Hagar M. Mohamed, Manohar Noone, Una Gibbons · World Journal of Advanced Research and Reviews · 2025
Aim: This study aims to evaluate the antioxidant capacity of black tea, turmeric, melatonin, and chamomile/lavender tea using the iodine clock reaction, a kinetic method for assessing antioxidant activity. Background: Oxidative stress, caused by an imbalance between free radicals and antioxidants, contributes to chronic diseases, including cardiovascular diseases and neurodegenerative disorders. Antioxidants play a crucial role in mitigating oxidative damage. Methods: The iodine clock reaction was utilized to assess antioxidant activity by measuring the delay in the oxidation process. Reaction times were recorded for each antioxidant sample, and statistical analysis (P-test) was performed to determine significance compared to the control. Results: Black tea exhibited the longest reaction delay (612.47s), indicating the highest antioxidant potential, followed by melatonin (344.75s), chamomile/lavender tea (248.98s), and turmeric (200.56s). Statistical analysis confirmed significant differences in antioxidant efficiencies (P < 0.05). Conclusion: The results suggest that black tea is the most potent antioxidant among the tested samples, supporting its health benefits. The iodine clock reaction proves to be a reliable method for antioxidant evaluation. Further research should explore additional antioxidants and optimize experimental conditions.