Antimicrobial, anti-inflammatory, anticancer and antiviral activity of bioactive compounds from Pseudomonas aeruginosa isolated from Mediterranean Sea, Alexandria, Egypt
Abdelraheem N. Mohamed, Abdel Kareem S. H. Mohamed, Asmaa Mohamed Zahran, Ahmed Mohamed Gad, Alsayed E. Mekky · Microbial Biosystems · 2025
In this study, four seawater and sediment samples were collected, Marine Pseudomonas aeruginosa (K3) was isolated from Alexandria, Egypt, and identified by using 16S rRNA gene sequencing and biochemical analyses. Optimization was carried out with various parameters to determine the optimum conditions to produce bio active compounds. Pseudomonas aeruginosa k3 is known for producing bioactive compounds that are effective against a variety of pathogenic microorganisms, including Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC 8739), Bacillus subtilis (ATCC 6633), Klebsiella pneumonia (ATCC 13883), Enterococcus faecalis (ATCC29212), Vibrio fluvialis (ATCC33809), and Pseudomonas aeruginosa (ATCC9027). GC-MS chromatography was used to identify the bioactive components. Additionally, we investigated the antiviral (HAV and CoxB4), anti-inflammatory (HRBC hemolysis and membrane stabilization), and anticancer (HepG2, Vero, and MCF-7) activity.