Obtaining High-resolution Images with a Large Field of View in Optical Microscopy
Ekaterina V. Andryushchenko, Vladimir Ivanovich Guzhov · 2024
Optical microscopy systems are widely used in various fields such as biology, medicine, and industry. The spatial resolution of a microscope is determined by the set of micro-lenses used. Due to their design features, there is an inverse dependence: the largest field of view is obtained when using a low magnification, low resolution objective lens. At the same time, lenses with lower resolution are cheaper due to the simplicity of manufacturing. Therefore, increasing the spatial resolution with such lenses is quite a promising direction of work. The aim of the paper is to improve the spatial resolution in optical microscopy by using objective lenses with large field of view. The enhancement of spatial resolution is accomplished using an analytical expression for the spectrum of discrete signals obtained using subpixel shifts. In this case, the spectrum of the function is supplemented by a multiplier, the type of which depends on the type of lens aperture. To obtain high-resolution features, it is necessary to divide the Fourier spectrum of an image obtained from several subpixel shifted images by a multiplier depending on the chosen aperture. This multiplier is called the aperture function. The aperture function is determined by the type of lens used and can be its passport value. The paper shows an experimental method for calibrating a lens (obtaining its aperture function) with a low resolution (10X) based on images acquired with lenses with a high resolution (100X).