Protocol to develop a chemotherapy drug screening process by constructing a cancer prognostic model using public databases
Weiyu Bai · STAR Protocols · 2024
Drug resistance is currently the biggest challenge in cancer chemotherapy. Here, we present a protocol to develop a chemotherapy drug screening process by constructing a cancer prognostic model (PM) using public databases. We describe steps for downloading code and data, preparing the expression matrix and metadata for analysis, screening modeling genes, and constructing a PM. We then detail procedures for constructing predictive websites for cancer patients’ survival based on their age, tumor stage, gene expression levels, and risk scores. For complete details on the use and execution of this protocol, please refer to Bai et al. 1 • Steps for building a cancer prognosis model • Instructions to build a website for cancer prognosis analysis • Technology for predicting chemotherapy drug sensitivity Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Drug resistance is currently the biggest challenge in cancer chemotherapy. Here, we present a protocol to develop a chemotherapy drug screening process by constructing a cancer prognostic model (PM) using public databases. We describe steps for downloading code and data, preparing the expression matrix and metadata for analysis, screening modeling genes, and constructing a PM. We then detail procedures for constructing predictive websites for cancer patients’ survival based on their age, tumor stage, gene expression levels, and risk scores.