Fasta file of Sanger Sequences
Deborah A. Neher, Thomas R. Weicht, Tucker D. Andrews · Figshare · 2022
AG groups were determined by Sanger sequencing of the internal transcribed spacer (ITS) region using the ITS1F (CTT GGT CAT TTA GAG G AAG TAA) and ITS4B (CAG GAG ACT TGT ACA CGG TCC AG) primer pair (758 bp) (Gardes and Bruns 1993). Reactions were held at 94°C for 3 min to denature the DNA, with amplification proceeding for 35 cycles at 94 °C for 45 s, 51 °C for 60 s, and 72 °C for 90 s; a final extension of 10 min at 72 °C. Contigs were assembled using CAP3 software (Huang and Madan 1999). Taxonomy was assigned to each OTU via the NCBI BLASTn database (https://blast.ncbi.nlm.nih.gov) with criteria as 0.0 E and nucleotide match of at least 97%. Gardes, M., and T. D. Bruns. 1993. ITS primers with enhanced specificity for Basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology 2:113-118. Huang, X., and Madan, A. 1999. CAP3: A DNA sequence assembly program. Genome Res 9:868-877.