Green Surfing vector maps
João Vitor Dutra Molino · Zenodo (CERN European Organization for Nuclear Research) · 2021
Overview Sequences were prepared in SNAPGENE and are the sequences used in the manuscript referenced below. Files info: Each construct has 3 correlated files. The .gb is the genbank format. The .dna is the SNAPGENE native format and the vector map is saved in a .png file. Construct pJPZ12: construct for expression of mVenus in the cytosol; pJPZ11: construct for expression and secretion of mVenus; pJPZ11_MAW8: construct with a truncated version of MAW8 that contained the GPI anchor signal; pJPZ11_HAP2: construct with a truncated version of HAP2 that contained the transmembrane domain; pJPZ11_GP1: construct with the mature hydroxyproline-rich GP1 protein. pJPZ11_GP1mCherry: construct with the mature hydroxyproline-rich GP1 protein and a mCherry fused to C-terminal. For more information on the constructs, check our paper. Consider citing our work. Development of a Cell Surface Display System in Chlamydomonas reinhardtii Molino et al. 2021