Knowledge-Based Image Analysis: Bayesian Evidences Enable the Comparison of Different Image Segmentation Pipelines
Mats Leif Moskopp, Andreas Deußen, Peter Dieterich · 2024
The analysis and evaluation of microscopic image data is essential in life sciences. Increasing temporal and spatial digital image resolution and the size of data sets promotes the necessity of automated image analysis. Previously, our group proposed a Bayesian formalism that allows for converting the experimenter’s knowledge, in the form of a manually segmented image, into machine-readable probability distributions of the parameters of an image segmentation pipeline. This approach preserved the level of detail provided by expert knowledge and interobserver variability and has proven robust to a variety of recording qualities and imaging artifacts. In the present work, Bayesian evidences were used to compare different image processing pipelines. As an illustrative example, a microscopic phase contrast image of a wound healing assay and its manual segmentation by the experimenter (ground truth) are used. Six different variations of image segmentation pipelines are introduced. The aim was to find the image segmentation pipeline that is best to automatically segment the input image given the expert knowledge with respect to the principle of Occam’s razor to avoid unnecessary complexity and computation. While none of the introduced image segmentation pipelines fail completely, it is illustrated that assessing the quality of the image segmentation with the naked eye is not feasible. Bayesian evidence (and the intrinsically estimated uncertainty σ of the image segmentation) is used to choose the best image processing pipeline for the given image. This work illustrates a proof of principle and is extendable to a diverse range of image segmentation problems.