MTT (Assay protocol v1

Abdulkareem Hameed Abd, Enas Jawad Kadhim, Matin Mahmood · 2023

MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)) is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. AMJ13: 2 (Ahmed.Murtuda ,Jabria 2013)cell line: The cell line of breast cancer has been obtained from Iraqi breast cancer which originated from the prime tumor of an old Iraqi woman (70 years) with a histological identification with carcinoma of infiltrating ductal (1). SK-GT-4:esophageal carcinomacell line was established from a primary tumors in 1989 from a 89 year-old Caucasian male who presented with dysphagia secondary to a well-differentiated adenocarcinoma arising in the Barrett epithelium of the distal oesophagus

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