effect of warfarin on tail bleeding time of male wistar rats model type 2 diabetes mellitus
Gusti Putu Satya Diva Pradana, I Putu Dedy Arjita, Musyarrafah Musyarrafah, Ety Retno Setyowati · International Journal of Health Sciences · 2022
Diabetes mellitus (DM) is a chronic metabolic disease that characterized by elevated blood glucose levels. Warfarin is an anticoagulant drug that is often used to treat and prevent microvascular and macrovascular complications. This study wanted to find out the effect of warfarin on the tail bleeding time of male Wistar rats with type 2 diabetes mellitus model. This study used quasi-experimental with posttest research design with an in vivo control group with male Wistar rats model DMT2 induced by nicotinamide and streptozotocin, the experimental group was given warfarin at a dose of 2 mg/Kg of body weight. The number of samples as many as 24 tails. The samples were divided into 4 groups, namely DM + Warfarin, DM + Aquades, Normal + Warfarin, Normal + Aquades. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests. The results of the average duration of tail bleeding time for each group is DM + Warfarin for 1200 ± 0.00 seconds, DM + Aquades for 913 ± 194.84 seconds, Normal group + Warfarin for 1173.3 ± 65.31 seconds, Normal + Aquades for 619.3 ± 319.64 seconds. The Kruskal-Wallis test showed that there was an effect of warfarin on tail bleeding time.