Removal of Leukocytes from Whole Blood and Erythrocyte Suspensions by Filtration through Cotton Wool
P. Diepenhorst, R. Sprokholt, H. K. Prins · Vox Sanguinis · 1972
(1) Sterile filtration of whole blood or blood cell suspensions in saline through columns of tightly packed cotton wool results in removal of over 95% of the leukocytes.(2) From whole blood, thrombocytes are inefficiently removed by the filtration procedure;filtration of blood cell suspensions in saline results in a better removal. (3) A system is designed by which filtration of 500 ml portions can be performed in less than 1 h at room temperature. (4) Red cell recovery with this system is over 90%. (5) Neither pyrogen tests nor toxicity tests did reveal the elution of harmful substances from properly prepared columns.