SIMPLE IN VITRO TESTING METHOD FOR ANTITHROMBOGENIC EVALUATION OF CENTRIFUGAL BLOOD PUMPS
Osamu Maruyama, Yosuke Tomari, Daisuke Sugiyama, Masahiro NISHIDA, Tatsuo Tsutsui, Takashi Yamane · ASAIO Journal · 2006
PURPOSE Our objective is to establish an evaluation system utilizing in vitro antithrombogenic testing in order to judge whether centrifugal blood pumps have sufficient antithrombogenicity to withstand animal experiments. METHOD In vitro tests using a mock circulation system were carried out using a monopivot (DD) pump and three types of hydrodynamic bearing (HH) pumps, under the conditions of 100 mmHg, 1.0–5.0 L/min, and ACT 150–400 for 2–10 hours at 37°. Purchased bovine blood was used for the test blood, and sodium citrate and calcium chloride were used to control ACT. In our previous study, it was found that a large amount of thrombus was generated during operation in one type of HH pump only 2 hours after it was connected to an ovine native heart, whereas a DD pump completed 7 days of animal experiments although small amounts of thrombus were seen in the pump. RESULTS Under the condition of ACT 200 for 2 hours, large amounts of thrombus were generated in the HH pumps, as had previously been seen in the animal experiments, whereas none was generated in the DD pump even though some thrombus had been found in the animal experiments. SUMMARY From the results obtained with the HH pumps, the establishment of an in vitro antithrombogenic testing method can be expected. In the future, centrifugal blood pumps having similar antithrombogenicity to the DD pump will also be able to be evaluated by refining the test conditions.