Superoxide Measurement
Hui‐Ming Gao · BIO-PROTOCOL · 2011
Superoxide dismutase (SOD)-inhibitable reduction of tetrazolium salt, WST-1, is used to measure extracellular superoxide free radical release from various immune cells such as macrophage, neutrophils, microglia (brain macrophage) after stimulation. This protocol has been developed and improved over the years by various researchers in Dr. Hong’s lab, especially Dr. Bin Liu., 超氧化物歧化酶(SOD) - 抑制性还原的四唑盐,WST-1,用于测量刺激后从各种免疫细胞如巨噬细胞,中性粒细胞,小胶质细胞(脑巨噬细胞)的细胞外超氧自由基释放。 这个协议已经由Hong博士实验室的各种研究人员,尤其是刘博士多年来开发和改进。