A Supplemental Guide to Building the optoPlate-96 v1
Mary J. Dunlop · 2021
The optoPlate-96 is a device that uses small LEDs to illuminate cell cultures in a 96-well plate. It was developed by Lukasz Bugaj and Wendell Lim. If you haven’t read the original optoPlate-96 paper and its Supplementary Information you should start there (Bugaj and Lim, Nature Protocols 2019). During the COVID-19 shutdown I made an optoPlate-96 in my basement over the course of many months. The protocol is detailed and awesome. But there are some things it doesn’t tell you, a few small typos, and several places where I just got confused. If you’ve never ordered a PCB or done reflow soldering, but are interested in giving it a try, this guide is for you.