MicroED: conception, practice and future opportunities
Tamir Gonen · Acta Crystallographica Section A Foundations and Advances · 2021
In 2013 we unveiled the method Microcrystal Electron Diffraction (MicroED) and demonstrated that it is feasible to determine high-resolution protein structures by electron crystallography of three-dimensional crystals in an electron cryo-microscope (CryoEM). The CryoEM is used in diffraction mode for structural analysis of proteins of interest using crystals that are a billionth the size of what is needed for x-ray crystallography. In this seminar I will describe the basics of this method, from concept to data collection, analysis and structure determination, and illustrate how MicroED can be used for high throughput drug discovery.