Amalgamation and Characterization of Copper Nanoparticles Alleviated With Stachytarpheta Cayennensis and Its Anti-cancer Activity in Both in Vitro and in Vivo Animal Model
Vidya Devanathadesikan Seshadri · Research Square · 2021
Abstract Melanoma is an extremely malignant skin cancer with a probability of metastasis and accountable for the mainstream skin associated mortality. In the present study, we described the novel usage of Stachytarpheta cayennensis mediated copper nanoparticles and its anti-cancer activity in both in vitro and in vivo model of skin cancer. The synthesis of Cu-NPs was confirmed using UV-absorbance peak values ranging from 325-345 nm. The size of the nanoparticles was around 90nm, as deduced by the dynamic light scattering study.Furthermore, transmission electron microscopy (TEM) established that the morphology of the copper particles. Cytotoxicity of Cu-NPs of Stachytarpheta cayennensis illustrates the toxicity level of Stachytarpheta cayennensis. Also, the anti-cancer potential of Cu-NPs was evaluated in A375 cells. In experimental animals, body biochemical parameters like SOD, CAT, GSH were diminished in DMBA induced animals while Cu-NPs treatment raised the levels of the aforementioned enzymatic antioxidants compared to the control animals. Additionally, cytotoxicity assay, mitochondrial membrane potential (MMP), cell adhesion analysis, and the estimation of reactive oxygen species (ROS) in the presence of Cu-NPs was evaluated by standard protocols. The present study's outcomes confirm the defensive and valuable effects of copper loaded Stachytarpheta cayennensis against DMBA induced skin melanoma, animal model.