SYSTEMATIC REVIEW ON THE COMPARISON OF PRACTICALITY OF H9C2 AND HL-1 CELL LINE INVITRO MODELS IN TESTING THE ROS SCAVENGING ACTIVITY OF AYURVEDIC CARDIOPROTECTIVE MEDICINES
Deepika Sl · International Journal of AYUSH · 2021
A new initiative “Global Hearts” launched by WHO aims to beat back the global threat of cardiovascular diseases – the leading cause of death worldwide. By analysing different cardiovascular diseases (CVDs) the main pathophysiology behind is; oxidative stress generated by the increased production of reactive oxygen species (ROS). The ROS scavenging activity of medicines can be tested through invitro research. H9C2 cell line and HL-1 cell line are popularly used invitro models for testing the cardiotoxic and cardioprotective study of medicines. The administration of antioxidants helps to scavenge these ROS and can be utilized as both preventive and curative aspect of cardiovascular diseases. In Ayurveda, many single drugs and polyherbal preparations are mentioned in hridroga chikilsa (treatment for heart diseases) with a natural antioxidant supply. Among them, some were tested through invitro studies. This systematic review tries to compare the practicality of H9C2 cell line and HL-1 cell line invitro models in testing the ROS scavenging activity of Ayurvedic cardioprotective medicines and this review was completed by the comprehensive analysis of published research works in PubMed database and google scholar. The review of the literature was arranged systematically, guided by the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) flow diagram. The review was concluded by comparing the number of available research works on both cell line invitro models used in the study of Ayurvedic cardioprotective medicines. The H9C2 cell line model was found to be more utilized for testing the ROS scavenging activity of Ayurvedic cardioprotective medicines. KEYWORDS: H9C2 cell line, HL-1 cell line, Invitro study, Reactive oxygen species, Ayurvedic medicine