Preliminary trial report on mutation effects of low-temperature plasma on Pinus armandii Franch.
Xin Pei-ya · Nanfang nongye xuebao · 2014
【Objective】Mutation effects of low-temperature plasma on P.armandii were studied to provide theoretical references and practical guide for improving plant heredity by plasma mutation.【Method】P.armandii seeds were treated and induced by 6 different levels of plasma(150,180,210,240,270 and 300 W),and then the germination rate of seeds and growth status of the seedling were measured(control:no mutation).When seedlings grew for 180 d,their height,ground diameter and growth coefficient of variation were determined to evaluate the effects of plasma on P.armandii growth.【Result】Seed germination rate in 180,210 and 240 W treatment was 70.67%,74.67% and 72.00%,respectively.Seed germination rate of the control was 57.33%,which was significantly different(P0.05).Seedling height was 8.90,8.32 and 8.20 cm,which was significantly different from the control(7.83 cm).The thickest ground diameter of 1.88 mm(in 210 W treatment) was significantly different from the control(1.62 mm)and the ground diameter in 300 W treatment(1.50 mm).Seed germination rate in 270 and 300 W treatment was 47.33% and 28.67% down by 10.00% and 28.66%.Seedling height was 7.11 and 7.14 cm,which was remarkably lower than the control.Seedling ground diameter in 300 W treatment was lower than the control and significantly different.Growth coefficient of variation of seedling height and ground diameter in 300 W treatment was the highest(28.43 and 24.67).【Conclusion】Plasma under 270 W promoted seed germination and seedling growth,while plasma above 300 W inhibited germination and seedling growth of P.armandii.P.armandii seedling height and growth coefficient of variation could be increased by low-temperature plasma(300 W),and P.armandii breeding is better in this way.