Investigation of Potential therapeutic value of Shuanghuanglian Chinese medicine based on network pharmacology for coronavirus pneumonia
Jiasheng Xu, Kaili Liao, Guanyu Zhang, Yiran Li, Weimin Zhou, Jiehua Qiu · 2020
Abstract Objective: The interaction network between coronavirus pneumonia and Shuanghuanglian was established to explore the potential therapeutic effect of the active ingredients of Shuanghuanglian on coronary virus pneumonia.Methods: Using the TCMSP database, the effective components of Shuanghuanglian were obtained by screening consistent with oral utilization，drug similarity and blood-brain barrier permeability thresholds, and the drug target prediction was performed.The SARS treatment target mining was performed through the GeneCards database, and the two data sets of therapeutic target and drug target were analyzed and the intersection was screened, and the wayne map was drawn.The intersection genes were used as potential therapeutic targets. Cytoscape 3.6 software was used to build a drug active ingredient-therapeutic target interaction network and analyze the active ingredient-therapeutic target point network Degree parameters to find important active ingredients and targets. Using DAVID and String databases to perform GO,KEGG enrichment analysis and protein interaction analysis on the intersection genes to find out the potential signal pathway of Shuanghuanglian against SARS.Results: 43 effective components against SARS were screened, includingcoptisine、Mandenol、Ethyl linolenate、phytofluene、wogonin、FORSYTHINOL、baicalein、Moslosooflavone、Panicolin, etc.A total of 115 intersection genes with coronavirus pneumonia were screened as important treatment targets: based on the protein interaction network, Shuanghuanglian's therapeutic targets for diseases were significantly enriched in 1763 GO and 133 KEGG signaling pathways; The main action pathways are: responses to steroid hormones and ketones, and development of the reproductive system, responses to lipopolysaccharides, Toxins, responses to bacteria and aging, and effects on epithelial cell proliferation.Conclusion: The active ingredients in Shuanghuanglian Chinese medicine compound can act on the disease-related target of coronavirus pneumonia and have potential therapeutic effects on coronavirus pneumonia.