Efficient generation of mouse models with the prime editing system
Yao Liu, Xiangyang Li, Siting He, Shuhong Huang, Chao Li, Yulin Chen, Zhen Liu, Xingxu Huang, Xiaolong Wang · Cell Discovery · 2020
Most genetic diseases in humans are caused by single-nucleotide mutations. Although genome editing with either the CRISPR-based cytosine base editor (CBE) 1 or the adenine base editor (ABE) 2 holds great promise for gene correction of C-to-T and A-to-G base substitutions in some genetic diseases 3 , 4 , both editors are useless for correction of other variants such as base transversion, small insertions and deletions (indels).