Trehalose Lipid Biosurfactant Reduced Cancer Cell Viability but Did not Affect the Isometric Contraction of Rat Mesenteric Arteries in vitro
Boris Kadinov, Biliana Nikolova, Iana Ch. Tsoneva, Severina Semkova, Lyudmila Kabaivanova, Daniela Z. Dimitrova · International Journal Bioautomation · 2020
Trehalose lipid biosurfactant from Nocardia farcinica strain is a naturally derived substance with potent anticancer activity. The increasing interest in naturally derived substances-based modality of cancer treatment requires investigations of the possible adverse effects of these substances, including the effects on vasculature. Therefore the present study was designed to investigate the effect of Trehalose lipid on isometric contraction of isolated rat mesenteric arteries. The contractile responses of arteries under Trehalose lipid was studied using wire myography for small blood vessels. The isometric contractions of rat mesenteric artery rings with intact endothelium were examined. The effect of this biosurfactant was assessed in arteries precontracted with 42 mM KCl as a vascular smooth muscle depolarizing stimulus. The results showed that Trehalose lipid (75 µM) failed to change high K+-induced contractions. The observed lack of effect of Trehalose lipid biosurfactant on the contractility of rat mesenteric arteries in vitro together with finding of reduced cancer cells viability makes it to be a suitable for potential medical application.