Sandwich ELISA Protocol v3
Sam Li · 2019
Cytokine ELISA Troubleshooting Tips: Solutions and Buffers: Solutions and Buffers:Do not use sodium azide in any buffers or solutions, as sodium azide inactivates the horseradish-peroxidase enzyme. Poor signal-to-noise ratio Poor signal-to-noise ratio:Try Capture Antibody at 1-10µg/ml (generally 2µg/ml).