Rapid Whole-Blood Cholinesterase Assay with Potential Use for Biological Monitoring During Chemical Weapons Disposal
Richard S. Halbrook, Carmen E Guzman, Karen J Wilkinson, Annetta P. Watson, Nancy B. Munro, Lee R. Shugart · Journal of AOAC International · 1992
Abstract A protocol Is described to determine whole-blood cholinesterase activity using an automated highspeed centrifugal analyzer. Throughput rate was increased 10-fold (from 6 to 60 samples/h), and precision, as measured by a reduction In Intra-allquot variation, was significantly Improved (from 4.7 to 3.0%) by using an automated system rather than a manual system. Similar cholinesterase activity was determined for samples analyzed by both the automated and manual protocols, and Interlaboratory comparison Indicated no change In accuracy. The lower limit of detection was estimated to be 0.10 μmol/mL/mln. The 10-fold Increase In throughput facilitates use of the automated protocol when many analyses must be performed in a limited time.