Growth of Human Cancer Cell Lines in Two Different Conditions
Shahed Sbeta, Sarah M. Bean, Jacob Harris · Digital Commons @ Butler University (Butler University) · 2018
Growing human cell lines is expensive. A critical component of cell growth media is Fetal Bovine Serum (FBS) which costs more than $1.00 per ml. A new product, FBEssence, claims to do the same job as FBS but at almost half the cost. We measured growth rates of four human cell lines: Caco2 (colon adenocarcinoma), Calu3 (lung adenocarcinoma), K562 (lymphoblast leukemia) and GM12878 (immortalized B-lymphocyte) grown in media containing either FBEssence (VWR) or FBS (Atlantic Biologicals). The experiments were conducted without knowing which condition - A or B - contained which additive.