Application of The Standardized Form Magnetite Nanoparticles (Icnb) in Creature Simple and Practical Method of Additive Modernization of Preservation Solutions for Red Blood Cells
Andrey Belousov · Journal of Anesthesia & Intensive Care Medicine · 2019
ActualityRed blood cells (RBCs) transfusion is a critical, life-saving treatment for severe anemia caused by disease or chemotherapy, or by blood loss due to trauma or major surgery.For several decades RBCs components have been prepared as concentrates suspended in nutrient additive solution, which preserves and extends the shelf-life of the RBCs component, allowing up to 6-7 weeks of refrigerated storage [1].Nevertheless, during storage RBCs undergo a complex and progressive accumulation of physicochemical changes, collectively referred to as the RBCs storage lesion [2,3].Recent clinical studies have identified RBCs transfusion as an independent risk factor for increased morbidities and mortalities in certain groups of patients, including trauma, cardiac surgery and the critically-ill (reviewed in [4-6]).Additionally, some of these studies have identified that older stored RBCs are more strongly implicated in poorer outcomes compared to fresher RBCs [6].In order to address these concerns, there is renewed interest to better understand the RBCs storage lesion and to find ways to ameliorate the deleterious effects of storage, thereby improving the quality, efficacy and safety of RBCs components for all transfusion recipients.While increased research effort is being directed to better understand the effects of storage on RBCs and the potential impact on transfusion outcomes [7], slower progress is being made in finding ways to deter the detrimental effects of the RBCs storage lesion.Over the past 15-20 years, research into the development of new additive solutions has focused on ways to maintain higher intracellular levels of ATP and 2.3-DPG during storage of RBC components [1].Despite the RBCs having been a favorite experimental model for cellular biologists and biochemists, RBCs storage research has repeatedly demonstrated that a lot of fundamental biology about RBCs is still not well understood.The complexity of the interrelationship between RBCs biochemistry, cytoskeletal structure and membrane properties have made it difficult to predict how RBCs will respond to different storage conditions.Exposure of RBCs to non-physiological storage environments has pointed to the existence of previously unknown biochemical mechanisms in RBCs, including apoptotic-like processes, ion and osmotic channels that behave differently than expected, exposure of new Abstract This study was devoted to the learning of the use of nanotechnology to correct the functional activity of red blood cells (RBCs) at the storage stages at a positive temperature.It was established that saline NaCl, which had previously been processed by magnetite nanoparticles (ICNB) had a marked membrane-stabilizing effect, inhibits hemolysis and increasing the sedimentation stability of preserved RBCs.The complex analysis of the obtained data allowed to determine the primary mechanisms effect of the saline NaCl, which had previously been processed by ICNB on the preserved RBCs.The proposed method of additive modernization of preserved RBCs was adapted to the production process.The optimization results were obtained in creating a simple and practical method of additive modernization of preservation solutions that does not violate the compliance requirements, improves the quality, efficiency and safety transfusion of RBCs.