In vitro and in vivo models used for antimalarial activity: A brief review
Mithun Rudrapal, Dipak Chetia · Asian Journal of Pharmacy and Pharmacology · 2019
In this review, the laboratory protocol commonly employed for the biological evaluation of antimalarial activity of new drug substances including natural products has been detailed in a generalized, but concise form.The evaluation strategy covers a systematic and standardized methodology starting from cell-based screening to the assay in vitro in vivo method using animal models.These assay methods primarily focus on blood/ erythrocytic stages of the Plasmodium parasite, either ( ) or ( ) since this particular stage of the parasite mainly causes in vitro P. falciparum in vivo P. yoelii symptoms, manifestations and associated pathogenesis of the disease.This is the only parasitic stage in malaria disease that can be maintained in continuous blood cultures and also remains to be the prime target for most of the antimalarial drug molecules.A general approach to the antimalarial screening ( and assay methods) routinely used for in vitro in vivo the sensitivity testing of newly developed antimalarial compounds is described herein.