Influence of temperature and storage time on reticulocyte parameters and establishment of reference values for laboratory parameters
Jiang Xing · Zhiye yu jiankang · 2013
[Objective]To study the influence on temperature and storage time on reticulocyte parameters,establish the reference range.[Methods]The specimens were stored at different temperature for different time,as so to observe the variations of reticulocyte parameters.[Results]Under the condition of low temperature(2-8 ℃),the reticulocyte count(Ret%),reticulocyte absolute value,high laser reticulocyte percentage(HLR%),high laser reticulocyte absolute value,immature reticulocyte fraction(IRF) and mean spherical corpuscular volume(MSCW) had not changed within 72 hours(P 0.05).Under the condition of room temperature(18-25 ℃),except that IRF had no obvious change within 48 hours,other parameters had changed significantly after 24 hours(P 0.05).The reference range from statistical results was wider than the setting reference range,but the pathological low limit was basically the same.[Conclusion]Reticulocyte specimens should be stored in low temperature(2-8 ℃),and the determination should be completed within 48 hours.