In Vitro Anticancer Activity of Ethanolic Extracts of Syzygium Aromaticum in HEp2 Cell Line
Shivanee Gupta · International Journal for Research in Applied Science and Engineering Technology · 2019
Cloves are the aromatic flower buds of a tree in the family Myrtaceae, Syzygium aromaticum.They show various medicinal properties, conventionally used asrespiratory and digestive ailments.Its medicinal properties are antioxidant, antiseptic, anti-helmintic, anesthetic, anti-inflammatory, rubefacient and anti-flatulent.It shows anti-cancerous property on various cancerous cell line such as HeLa cell, lungs cancer, prostate cancer, breast cancer, esophageal cancer.Ethanolic extract of clove has been taken to show anti-cancerous effect on HEp2 cell line.Different concentration of clove extract has been taken to show effect on HEp2 cell line, 1000 µg/ml, 100 µg/ml, 10 µg/ml and 1 µg/ml shows cytotoxicity on HEp2 cell line.Least cytotoxic effect was observed in 1 µg/ml and highest in 1000 µg/ml.thus cytotoxic effect was observed in all concentrations but the effect is varying different doses.This cytotoxicity was determined by performing MTT Assay, NRU Assay and DNA Fragmentation Assay.All shows cytotoxic effect on 1000 µg/ml but there is varying result in MTT and NRU assay but the effect properly observed in DNA fragmentation assay.DNA Fragmentation assay indicated the apoptotic cell death at all doses.Thus it can be concluded that clove have cytotoxic effect on HEp2 cell line.