Biological activity of antioxidant compounds in monocytes THP-1
Jan Hájek · Digital Repository (National Repository of Grey Literature) · 2010
Charles University in Prague University Roma Tre Faculty of Pharmacy in Hradec Králové Department of Biology Department of Biochemical Sciences Candidate: Jan Hájek Supervisor: PharmDr. Iva Boušová, Ph.D. Prof. Sandra Incerpi, Prof. Jens Z. Pedersen Title of diploma thesis: Biological activity of antioxidant compounds in monocytes THP-1 Elaboration of this work is based on the experiments carried out in the Laboratory of Physiology at University Roma Tre in Rome, under the supervision of Prof. Sandra Incerpi. The team of this laboratory studies the antioxidants of different structures and evaluates their ability to prevent the production of reactive oxygen species (ROS). These reactive species are essential for many physiological processes, but they can also have deleterious effects and participate in the development of various diseases. In this work, I dealt with a study of antioxidant properties of different polyphenolic compounds (mosloflavone, negletein, 5,6-dihydroxyflavone, baicalein, gallic acid, and compound 1625) in the cell culture of human monocytes THP-1 and also in the in vitro test. Three different methods were used: intracellular ROS determination (dichlorofluorescein assay), cytotoxicity assay, and electron paramagnetic resonance spectroscopy (EPR). Mosloflavone showed scavenging...