Effect of Methyl- Propyl (MP), Methyl- Butyl (MB), and Methyl-Propyl-Butyl (MPB) mixtures on the proliferation, DNA fragmentation and estradiol secretion of MCF-7 breast cancer cells and MCF-10A normal breast epithelial cells
Ewa Łucja Gregoraszczuk · Jagiellonian University Repository (Jagiellonian University) · 2017
Since parabens are used as preservatives in cosmetics as mixtures, here we aimed to compare the effects of Methyl-Propyl (MP), Methyl-Butyl (MB), and Methyl-Propyl-Butyl (MPB) on MCF-7 (breast cancer cells) and MF-10A (non-cancer breast epithelial cells) proliferation (Alamar Blue assay), DNA fragmentation ( ELISA), and estradiol (E2) secretion (ELISA).Cells were exposed to mixtures at a concentration of 20 N, either alone or supplemented with parabens together with 10 nM 17β-estradiol.ICI 182,780 (100 nM) was used to demonstrate the involvement of Estradiol Receptor (ER) in the action of the paraben mixture.For the MCF-7 cells, all mixtures increased cell proliferation and inhibited DNA fragmentation.No synergistic action with E2 was observed, and only the MPB increased E2 secretion by MCF-7 cells.For the MCF-10A cells, none of the mixtures affected cell proliferation, and only MPB decreased DNA fragmentation.No mixture affected the secretion of estradiol in either cell line.The ICI 182,780 inhibitory effect on parabens stimulated cell proliferation only in ER+ cells, thereby confi rming their ER-mediated action.In conclusion, based on the presented results we suggest that simultaneous application of more than one paraben might promote the progression of malignancy but not initiation of cancer.