Directing Drug Evolution
Sarah Webb · BioTechniques · 2017
DIRECTING DRUG EVOLUTIONDrug discovery research comes in two primary flavors.First there is the traditional medicinal chemistry approach where chemists use specialized reactions to carefully design and synthesize small molecule drug candidates that can then be tested against defined molecular targets.The second approach involves the use of directed evolution techniques, which have recently propelled drug discovery efforts for large molecules such as therapeutic antibodies.But in between these two approaches, there exists a vast range of "drug space" that researchers haven't been able to address, says medicinal chemist Andrew Davis of AstraZeneca in Mölndal, Sweden (1).Up to 80 percent of drug targets may actually reside inside cells rather than on their surfaces.These are targets involved in complex protein interactions or involve specialized proteins such as phosphatases that are currently considered undruggable. R Eliminate the Guesswork in CRISPR Gene Editing Detection aati-us.com Accurate detectionWith T7 Endonuclease I Visualize results With high sensitivity detection Characterize mutationsThrough zygosity identification Confidently identify CRISPR modifications with the AccuCleave T7 kits.Available for both agarose gels and the Fragment Analyzer.aati-us.com aati-us.com