Molecular Characterization of Antimicrobial Drug Resistance in Escherichia coli Isolated from Clinical Samples
Ibtisam Habeeb Al-Azawi, Aqeel Reheeum Hassan, Alaa Hamza Jaber · International Journal of Current Pharmaceutical Review and Research · 2017
A total of 49 different clinical samples (urine n=30, stool n=10, and blood n=8) were collected from patient admitted to the Al-Sadder medical City in Al-Najaf Governorate-Iraq. The results demonstrated that 49 specimens (100%) were diagnosed as E. coli by cultural, biochemical characteristics and Vitek2® system. Polymerase Chain Reaction has been used to detect of some genes which coding antimicrobial resistance in E. coli isolates. Regarding genes that responsible for ESBL enzymes (blaCTX-M, blaOXA and blaTEM), the current results proved that blaTEM genes have highest rate (97.95%) followed by blaTEM and blaOXA (93.75%) for each.