Grayscale Conversion of Histopathological Slide Images as a Preprocessing Step for Image Segmentation
Jashojit Mukherjee, Indra Kanta Maitra, Kashi Nath Dey, Samir Kumar Bandyopadhyay, Debnath Bhattacharyya, Tai-hoon Kim · International Journal of Software Engineering and Its Applications · 2016
This paper presents a method of conversion of color image of histopathological slides to grayscale as a preprocessing step for image segmentation. The method utilizes characteristics of these images to reduce the amount of information lost during the conversion. It is a global mapping scheme and the same color does not map to two different grayscale values. Color information is added to the brightness information to reduce information loss and the weights for the added color are dependent on the distribution of the pixels in a particular image. The method was tested on a large set of histopathological slide images and convincing results were obtained some of which has been published in this paper.