Colloidal gold reaction : a contribution to serology
Robert G. Bannerman · ERA · 1919
The Thesis is an attempt, in the first place ,| to assess the value of the Colloidal Gold reaction as a diagnostic procedure, and in the second place, to gain some insight into the nature of the reaction as a 1 serological phenomenon.In 1912, Lange, of Vienna, was making use of | .(2) An analysis of the cases examined: these number 12(3) Researches into the nature of the reaction, lead¬ ing to a suggested explanation of some features.(4) Conclusions.|^> A Bibliography and two appendices are added, References to Bibliography in red ink.! 3 TECHNIQUE of the TEST.There are considerable technical difficult¬ ies in the preparation of suitable colloidal gold ■ solution, which is the essential reagent m the test.Numerous methods of preparation of colloid¬ al gold have been practised, starting perhaps with the aurum potabile of the alchemists: Mme.Fulhame in IE94, experimented with such solutions, and the sub¬ ject was much enlightened by the researches of Fara¬ day , Bernelius, Davy, and Bredig: Faraday laid stress on the necessity for clean glass in all the manipulat¬ ions.In 1898, Zsigmondy made an advance relevant to j j • the present reaction when he elaborated a method for the preparation of colloidal gold solutions which con-i i J sists essentially in the reduction of gold chloride in alkaline solution by formalin, or certain other organic re a. ents: for this procedure to succeed, a high standard of purity of distilled water has to be reached.Many other reagents than formalin have been used: one of these which has been tried by the writer * (»&■<>) is hydrazin hydrate, as used in Gutbier's method:with this method, the quantity of the inducing reag¬ ent and the alkalinity of the gold chloride solution determine the colour obtained: in the present exper¬ iments solutions of good colour were readily obtained,, but were found to be quite unsuitable xbr the react¬ ion: the reason of &his failure is that m making j gold solution by the hydrasin method, a considerable I For a supply of this, the writer is indebted to