1PT167 Multidimensional free energy landscape of folding of DHFR(The 50th Annual Meeting of the Biophysical Society of Japan)
Tomoki P. Terada, Masaki Sasai · Seibutsu Butsuri · 2012
GeneralIncorporatedAssociation cettoida] probe atomic force microscopy (AFM).KinA and SpoOF were immobitized on a glass substrate and a glass sphere using Langmuir B]odget films of a chelate amphiphile.The conditions of adsorbing proteins were determined using quartz crystal microbalance and AFM.Surface fbrce protltes for KinA and SpoOF showed only repulsive force in the buffer sotution with/without ATP, Adding cefactor Mg2+ and Ca2+ to buffer so]ution with