2P156 Rotation Measurement of Na^+-driven Chimeric Flagellar Motor with Tandem PomA Mutants(11. Molecular motor,Poster)
Yong‐Suk Che, Yoshiyuki Sowa · Seibutsu Butsuri · 2013
Mycoplasma mobile, a fish pathogen, glides on solid surfaces with a unique mechanism.In this mechanism, Gli521 (521kDa) transmits movements from motor to leg as "crank", and Gli349 (349kDa) attaches to solid surface and pull cell body as "leg".Here, we analyzed molecular shapes of these proteins by negative-staining electron microscopy and high speed AFM.Gli521 molecule is 120 nm long and shaped "ω", featured with a central hinge, at which the molecule bends only to one direction.Gli349, known as 100 nm " 8th note ", shaped molecule by rotary shadowing TEM was divided into small and large domains connected by a thin, elastic filament.We will discuss the movements of molecules in the gliding mechanism.