Protocol Modification To Determine The Cytotoxic Potential Of Drugs Using Cell Viability Assays That Rely On The Reducing Property Of Viable Cells
Niraj Konchady Shenoy, Niraj Konchady Shenoy, Xiaosheng Wu, Thomas Elmer Witzig · Protocol Exchange · 2017
The CellTiter-Blue ® Cell Viability Assay provides a homogeneous, fl uorometric method for estimating the number of viable cells present in multiwell plates.It uses the indicator dye resazurin to measure the metabolic capacity of cells-an indicator of cell viability.Viable cells retain the ability to reduce resazurin into resorufi n, which is highly fl uorescent (Figure 1).Nonviable cells rapidly lose metabolic capacity, do not reduce the indicator dye, and thus do not generate a fl uorescent signal.The CellTiter-Blue ® Reagent is a buff ered solution containing highly purifi ed resazurin.The ingredients have been optimized for use as a cell viability assay.The spectral properties of CellTiter-Blue ® Reagent change upon reduction of resazurin to resorufi n (Figure 2).Resazurin is dark blue in color and has little intrinsic fl uorescence until it is reduced to resorufi n, which is pink and highly fl uorescent (579 Ex /584 Em ).