Chemically induced strong cellular hypertrophy often reduces the accuracy of cytotoxicity measurements obtained using the ATP assay
Haruna Tahara, Yusuke Yamamoto, Shun Matsuda, Hiroe Yoshizawa, Masaharu Fujita, Takanori Hioki, Toshihiko Kasahara · The Journal of Toxicological Sciences · 2017
> 0.8, at least 2 out of 3 experiments), and the cell size was effectively the same as that of the controls. We concluded that the poor correlation between the two methods was attributable to an increase in the content of intracellular ATP because of the chemically induced cell and mitochondrial hypertrophy. We showed that the ATP assay is unsuitable for assessing the cytotoxicity of compounds that induce cell hypertrophy with increase in the mitochondrial area and ATP content.