Grid-based visual aid for enhanced microscopy screening in diagnostic cytopathology
Christos Riziotis, Evangelos Tsiambas · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · 2016
A grid acting as a spatial reference and calibration aid, fabricated on glass cover slips by laser micromachining and attached on the carrier microscope slide, is proposed as a visual aid for the improvement of microscopy diagnostic procedure in the screening of cytological slides. A set of borderline and also abnormal PAP test cases -according to Bethesda 2014 revised terminology- was analyzed by conventional and grid based screening procedures, and statistical analysis showed that the introduced grid-based microscopy led to an improved diagnosis by identifying an increased number of abnormal cells in a shorter period of time, especially concerning the number of pre- or neoplastic/cancerous cells.