Big EP: automated structure solution pipeline deployment at Diamond Light Source
Irakli Sikharulidze, Graeme Winter, David R. Hall · Acta Crystallographica Section A Foundations and Advances · 2016
A new data acquisition method was developed using an ultrasonic acoustic levitator and a next-generation pixel array detector in order to collect dataset rapidly at room temperature.Spinning of crystals in the levitated liquid droplet caused by the acoustic streaming and a rapid frame rate of few thousand Hz with the Eiger X detectors allow us to collect a dataset less than few hundred millisecond.We conducted the proof of principle experiments using lysozyme crystals and demonstrated the successful solution of the crystal structure.No damage due to the acoustic pressure was observed.Diffraction experiments were set up at the beamline X06SA, Swiss Light Source, Paul Scherrer Institut.Diffraction images were collected at 133 Hz with the Eiger X 16M detector and processed by CrystFEL data processing suite because of a smooth transition of rotation axis during the exposure.Details of the diffraction experiments, comparisons of structures solved by different methods and possible applications with this method will be presented.