Evaluation of Emit Qst assay with the use of tobramycin, gentamicin and amikacin.
Teruaki Kitaura, Hiroshi Fukuchi, SATSUKI TSUKIAI, YOSHIE WADA · Japanese Journal of Hospital Pharmacy · 1986
Evaluation was made on the newly developed Emit Qst assay with the use of tobramycin (TOB), gentamicin (GM), and amikacin (AMK). Items examined were stability and accuracy of the standard curve, reproducibility, and correlation with Emit assay.The standard curves of TOB, GM and AMK prepared by a calibrator for Emit Qst assay were all stable for at least ten weeks. As for reproducibility, the coefficients of variation were all lower than 10% at all concentrations except for GM at 1μg/ml whose coefficient of variation was 21.7%. A high correlation was observed between Emit Qst assay and Emit assay with r=0.993 for TOB, r=0.997 for GM, and r=0.976 for AMK.It was confirmed that in cmparison with Emit assay, Emit Qst assay has a higher applicability in making determination in an emergency and of a small number of samples having no need to prepare a standard curve on each occasion, and is superior economically with the loss of the reagent being smaller.