VISUALIZATION AND RESOLUTION IN LOCALIZATION MICROSCOPY
Robert P. J. Nieuwenhuizen, Sjoerd Stallinga, Bernd Rieger · 2015
camera together with the magnification of the objective lens naturally define the way how an image is sampled. Localization microscopy lacks any natural ways of visualization, it is an important issue how data should be visualized. Basically, the positions of single fluorescent emitters are estimated from the asynchronous recordings of blinking emitters. To this end, different localization schemes are employed that estimate the positions (i.e., a list of coordinates), as well as the estimated fluorophore intensities, background intensities, localization precisions, and possibly other parameters depending on the localization method. Thus, localization microscopy produces data sets but no images initially. In literature a few methods are used on an ad-hoc basis, i.e. histogram binning, Gaussian rendering, jittering, Delaunay triangulation, and quad-tree visualization.