The influence of Romanowsky-Giemsa type of stains on the nuclear texture of desoxyribonuclease-treated cytological preparations.
Dietrich H. Wittekind, E. K. W. Schulte, V Kretschmer · PubMed · 1989
It had been observed that cell films where DNA had been partially digested by DNase stained differentially by the standard RG stain and a representative commercial Giemsa mixture. The reasons for this divergence were experimentally investigated. The lower concentration of Azure B in the commercial Giemsa mixture as compared with the standard RG stain emerged as the most likely factor causing the divergence of staining behaviour. A correlation of this assumption to the theory of the RG stain is tentatively suggested.