Example of an incomplete homologous recombination event detected by MLPA
Petra Langerak, Nygren, Anders O. H., Schouten Jan P., Jacobs Heinz · Figshare · 2011
Copyright information:Taken from "Rapid and quantitative detection of homologous and non-homologous recombination events using three oligonucleotide MLPA"Nucleic Acids Research 2005;33(22):e188-e188.Published online 9 Dec 2005PMCID:PMC1310898.© The Author 2005. Published by Oxford University Press. All rights reserved Representation of an incomplete homologous recombination event observed for the conditional inactivation of the PCNA gene. In green: the puroΔtk selection marker. Incomplete recombination is revealed by the absence of one of the two LoxP recombination sites (L3). The MLPA probe mix contained 12 control probes (C) and two probes specific for the 5′ and 3′ wild-type and LoxP recombination sites. Only part of the CE pattern is shown. In blue the CE pattern of untargeted ES cell clone, in red the CE pattern of an ES cell clone with an incomplete integration.