Inhibitory effect of Bridelia ovata Decne extract on HepG2 cell migration and invasion stimulated by fibroblast-conditioned media
Hifza Baig, Penchatr Diskul‐Na‐Ayudthaya, Churat Weeraphan, N. Monique Paricharttanakul, Jisnuson Svasti, Chantragan Srisomsap · 2015
Migration and invasion are the critical step during tumor metastasis. Therefore, one of the goals for cancer treatment is to suppress the migration and invasion of cancer cells. Here, the effect of Bridelia ovata Decne (BO) extract on cell migration and invasion was investigated using human liver cancer cell lines (HepG2) as a model system. Crude ethanol extract is obtained from plant extract using speed vacuum apparatus. The cytotoxic activities of ethanolic extract on HepG2 cell line were evaluated using 3-[4, 5-Dimethyl-2- thiazolyl]- 2, 5-diphenyltetrazolium bromide (MTT) assay. In vitro migration and invasion assays were performed using a modified Boyden chambers. The results showed that at 80% survival (IC20) with 47 μg/mL as concentration of BO extract inhibited invasion and migration of HepG2 cells. It could suggest that BO extract might be involved in the cytotoxic activity against HepG2 carcinoma cell.