Construction of hTCF_4 Yeast Two-Hybird Vector
Lin Gui · Journal of Weifang Medical College · 2002
Objective To investigate the coactavitor and corepressor in nuclei of hTCF 4,which is the important molecule of Wnt/Frizzled signaling pathway.Method We got the TCF 4 ND fragment from pCDNA TCF 4 by polymercase reaction(PCR).After digestion by Sai I and Not I,TCF 4 ND fragment were subcoloned into Yeast Two Hybird vector pPC86 and pDBleu.Result At last,hTCF 4 yeast two hybird vectors pPC86 TCF 4 and pDBleuTCF 4 were constructed and confined by RE digestion.Conclusion The construction of pPC86TCF 4 and pDBleuTCF 4 are helpful to the studying of Wnt/Frizzled pathway.