Rapid Detection of Contents of Alkaloids and Water from Radix Aconiti Preparata by Near-infrared Diffuse Reflectance Spectroscopy
Yaosheng Tu · Guangzhou Zhongyiyao Daxue xuebao · 2013
Objective To make a rapid analysis of the constituents of Radix Aconiti Preparata(RAP) by nearinfrared diffuse reflectance spectroscopy(NIDRS).Methods The NIDRS data of 119 RAP samples was collected for the establishment of quantitative analysis models of monoester alkaloids,diester alkaloids and water content NIDRS information by partial least squares(PLS) regression method.Results The modeling spectral ranges of RAP monoester alkaloids,diester alkaloids,and water content were 8 431.26-4 075.93,5 086.76-4 088.64,7 725.44-5 924.25,and 7 934.87-4 088.64 cm-1 respecitvely.The root mean square error of calibration(RMSEC) for monoester alkaloids,diester alkaloids,and water content was 0.098,0.034,and 0.002,and the correlation coefficient(r) of their calibration models was 0.955 9,0.969 4,and 0.976 7,respectively.The root mean square error of prediction(RMSEP) for monoester alkaloids,diester alkaloids,and water content was 0.168,0.039,and 0.004,and the correlation coefficient of their validation models was 0.926 6,0.982 6 and 0.836 3,respectively.The correlation coefficient of the predicted value with the reference value of monoester alkaloids,diester alkaloids,and moisture was 0.970,0.993 and 0.918,respectively(P0.001),indicating that NIDRS had the similar effect to high pressure liquid chromatography(HPLC) for the assay of alkaloids,a nd NIDRS was as good as oven drying method for the assay of water content.Conclusion NIDRS has the advantages of rapid analysis and accurately predicting results,without destroying the samples or complex pre-processing,which can be used for the prediction of corresponding ingredients of RAP.